Efficient and Scalable Process to Produce Novel and Highly Bioactive Purified Cytosolic Crystals from Bacillus thuringiensis.
Chicca J, Cazeault NR, Rus F, Abraham A, Garceau C, Li H, Atwa SM, Flanagan K, Soto ER, Morrison MS, Gazzola D, Hu Y, Liu DR, Nielsen MK, Urban JF Jr, Ostroff GR, Aroian RV.
Chicca J, et al.
Microbiol Spectr. 2022 Aug 31;10(4):e0235622. doi: 10.1128/spectrum.02356-22. Epub 2022 Aug 10.
Microbiol Spectr. 2022.
PMID: 35946940
Free PMC article.
Here, we address this severe limitation and present scalable and practical methods for large-scale purification of potently bioactive B. thuringiensis crystals and crystal proteins. The resultant product, called purified cytosolic crystals (PCC), is highly compatible with …
Here, we address this severe limitation and present scalable and practical methods for large-scale purification of potently bioactive B …